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immunostaining  (Vector Laboratories)


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    Vector Laboratories immunostaining
    Immunostaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+vectastain+abc+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Mouse+IgG)/pmc12886486-41-0-23
    Average 96 stars, based on 1142 article reviews
    immunostaining - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..

    Immunohistochemistry:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..

    Article Title: Stem cell markers in oral and oropharyngeal squamous cell carcinomas in relation to the site of origin and HPV infection: clinical implications
    Article Snippet: .. The IHC Vectastain ® Abc Kit (Vector Laboratories, Inc., Burlingame, CA) was used according to the manufacturer’s protocol. ..

    Article Title: Implications of Senescent Cell Burden and NRF2 Pathway in Uremic Calcification: A Translational Study
    Article Snippet: .. Immunohistochemistry (IHC) (Vectastain ABC kit, Vector Labs, Newark, CA, United States) and immunofluorescence (IF) were performed on TAs and EAs with anti-p16 Ink4a , anti-p21 Cip1 , anti-NRF2, anti-γH2AX, anti-osteopontin (OPN), anti-RUNX family transcription factor 2 (Runx2) and anti-alpha smooth muscle actin (α-SMA) primary antibodies and appropriate secondary antibodies. .. Fluorescence was observed with Axio-imager Z Apotome (Zeiss, Jena, Germany) using Montpellier MRI imaging platform or Axio-observer Z1 microscope (Zeiss, Jena, Germany).

    Article Title: Implications of Senescent Cell Burden and NRF2 Pathway in Uremic Calcification: A Translational Study.
    Article Snippet: .. Immunohistochemistry (IHC) (Vectastain ABC kit, Vector Labs, Newark, CA, United States) and immunofluorescence (IF) were performed on TAs and EAs with anti-p16Ink4a, anti-p21Cip1, anti-NRF2, anti-γH2AX, anti-osteopontin (OPN), anti-RUNX family transcription factor 2 (Runx2) and anti-alpha smooth muscle actin (α-SMA) primary antibodies and appropriate secondary antibodies. .. Fluorescence was observed with Axio-imager Z Apotome (Zeiss, Jena, Germany) using Montpellier MRI imaging platform or Axio-observer Z1 microscope (Zeiss, Jena, Germany).

    Article Title: Stem cell markers in oral and oropharyngeal squamous cell carcinomas in relation to the site of origin and HPV infection: clinical implications
    Article Snippet: .. The IHC Vectastain® Abc Kit (Vector Laboratories, Inc., Burlingame, CA) was used according to the manufacturer’s protocol. ..

    Staining:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..

    FACS:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..

    Immunofluorescence:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..

    Article Title: Implications of Senescent Cell Burden and NRF2 Pathway in Uremic Calcification: A Translational Study
    Article Snippet: .. Immunohistochemistry (IHC) (Vectastain ABC kit, Vector Labs, Newark, CA, United States) and immunofluorescence (IF) were performed on TAs and EAs with anti-p16 Ink4a , anti-p21 Cip1 , anti-NRF2, anti-γH2AX, anti-osteopontin (OPN), anti-RUNX family transcription factor 2 (Runx2) and anti-alpha smooth muscle actin (α-SMA) primary antibodies and appropriate secondary antibodies. .. Fluorescence was observed with Axio-imager Z Apotome (Zeiss, Jena, Germany) using Montpellier MRI imaging platform or Axio-observer Z1 microscope (Zeiss, Jena, Germany).

    Article Title: Implications of Senescent Cell Burden and NRF2 Pathway in Uremic Calcification: A Translational Study.
    Article Snippet: .. Immunohistochemistry (IHC) (Vectastain ABC kit, Vector Labs, Newark, CA, United States) and immunofluorescence (IF) were performed on TAs and EAs with anti-p16Ink4a, anti-p21Cip1, anti-NRF2, anti-γH2AX, anti-osteopontin (OPN), anti-RUNX family transcription factor 2 (Runx2) and anti-alpha smooth muscle actin (α-SMA) primary antibodies and appropriate secondary antibodies. .. Fluorescence was observed with Axio-imager Z Apotome (Zeiss, Jena, Germany) using Montpellier MRI imaging platform or Axio-observer Z1 microscope (Zeiss, Jena, Germany).

    Fluorescence:

    Article Title: Dysadherin awakens mechanical forces and promotes colorectal cancer progression
    Article Snippet: .. List of antibodies used for immunoblot analysis, co-IP, IHC, and IF staining Target Conjugate Catalog # (company/provider) Application Mouse Ki-67 - MA5-14520 IHC Mouse cleaved-Caspase 3 - 9661S (Cell Signaling Technology) IHC Human Dysadherin - M53 (Dr. Ino) IHC VECTASTAIN® ABC Kit, Rabbit IgG - PK-6101 (Vector Laboratories) IHC VECTASTAIN® ABC Kit, Mouse IgG - PK-6102 (Vector Laboratories) IHC Human Dysadherin - M53 (Dr. Ino) Immunoblot Human E-cadherin - 14472S (Cell Signaling Technology) Immunoblot Human β-actin - A5316 (Sigma-Aldrich) Immunoblot Human Fibronectin - ab2413 (Abcam) Immunoblot Human GAPDH - 5174S (Cell Signaling Technology) Immunoblot Human p-FAK(Y397) - 700255 (ThermoFisher) Immunoblot Human total FAK - 3285S (Cell Signaling Technology) Immunoblot Human Pan-cadherin - 4068S (Cell Signaling Technology) Immunoblot Human YAP - ab205270 (Abcam) Immunoblot Human p-YAP (S217) - ab76252 (Abcam) Immunoblot His-Tag - MA1-21315 (ThermoFisher) Immunoblot 46 Goat anti-mouse, Light chain-specific HRP 91196S (Cell Signaling Technology) Immunoblot Goat anti-mouse HRP 554002 (BD PharmigenTM) Immunoblot Goat anti-rabbit HRP 554021 (BD PharmigenTM) Immunoblot Annexin V FITC 556547 (BD PharmigenTM) FACS Human Dysadherin - M53 (Dr. Ino) IF Human Dysadherin APC M53 (Dr. Ino) IF Human active integrin β1 (12G10) - ab202641 (Abcam) IF Human Fibronectin - ab2413 (Abcam) IF Human EpCAM - 93790S (Cell Signaling Technology) IF Human EpCAM Alexa488 14-9326-82 (eBioscienceTM) IF Human F-actin Alexa555 A34055 (ThermoFisher) IF Human p-FAK(Y397) - 700255 (ThermoFisher) IF Human Paxillin - AHO0492(ThermoFisher) IF Human YAP - 14074 (Cell Signaling Technology) IF Mouse active integrin β1(9EG7) - 550531 (BD PharmigenTM) IF Mouse Dysadherin - SC-30606 (SantaCruz) IF Mouse Fibronectin - ab2413 (Abcam) IF Mouse a-SMA - A2547 (Sigma-Aldrich) IF Mouse EpCAM - 93790S (Cell Signaling Technology) IF Mouse p-FAK(Y397) - 700255 (ThermoFisher) IF 47 Human Myosin IIa - 3403 (Cell Signaling Technology) Immunoblot & IF Human Phosphor-Myosin IIa (Ser1943) - 14611 (Cell Signaling Technology) Immunoblot & IF His-tag - MA1-21315 (ThermoFisher) IF Donkey anti-mouse Alexa488 A21202 (ThermoFisher) IF Donkey anti-rabbit Alexa555 A31572 (ThermoFisher) IF Donkey anti-goat Alexa488 A11055 (ThermoFisher) IF Goat anti-mouse Alexa555 A21422 (ThermoFisher) IF Donkey anti-rat Alexa555 A48270 (ThermoFisher) IF Donkey anti-rabbit Alexa488 A21206 (ThermoFisher) IF Phalloidin Alexa555 A34055 (ThermoFisher) IF IHC, immunohistochemistry; IF, immunofluorescence; FACS, fluorescence-activated cell sorting; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; APC, adenomatous polyposis coli 48 Table S4. ..



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    ihc kit - by Bioz Stars, 2026-10
    96/100 stars
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    vectastain abc hrp dab ihc detection kit  (Vector Laboratories)
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    Vector Laboratories vectastain abc hrp dab ihc detection kit
    Persistent periodontal defects in Hyp groups despite Spp1 ablation. (A) H&E staining shows thin dentin (arrows) and wide pulp chamber compared to WT and Spp1 −/− controls. (B) Picrosirius red (PR) staining demonstrates that all Hyp groups have disorganized and detached PDL fibers at root surfaces (asterisks), compared to controls. (C) Immunohistochemistry <t>(IHC)</t> for osteopontin (OPN) shows accumulation of the protein around the osteocytes in alveolar bone (triangles) of Hyp and Hyp;Spp1 −/+ . Absence of OPN is apparent in Spp1 −/− and Hyp;Spp1 −/− mice. The graph in (D) IHC specific to bone sialoprotein (BSP) reveals unmineralized osteoid areas (asterisks) of alveolar bone that remained unchanged in Hyp groups despite Spp1 −/− deletion. (E) Acellular cementum thickness remains diminished in all Hyp mice compared to controls. (F) Dynamic mechanical analysis testing reveals compromised dynamic stiffness (K * ) and energy dissipation (tan δ) in Hyp mice remain despite Spp1 ablation. AB: alveolar bone; CC: cellular cementum; DE: dentin; PDL: periodontal ligament; AC: acellular cementum. * p < .05; ** p < .01; *** p < .001; **** p < .0001.
    Vectastain Abc Hrp Dab Ihc Detection Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vectastain abc hrp dab ihc detection kit - by Bioz Stars, 2026-10
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    Persistent periodontal defects in Hyp groups despite Spp1 ablation. (A) H&E staining shows thin dentin (arrows) and wide pulp chamber compared to WT and Spp1 −/− controls. (B) Picrosirius red (PR) staining demonstrates that all Hyp groups have disorganized and detached PDL fibers at root surfaces (asterisks), compared to controls. (C) Immunohistochemistry (IHC) for osteopontin (OPN) shows accumulation of the protein around the osteocytes in alveolar bone (triangles) of Hyp and Hyp;Spp1 −/+ . Absence of OPN is apparent in Spp1 −/− and Hyp;Spp1 −/− mice. The graph in (D) IHC specific to bone sialoprotein (BSP) reveals unmineralized osteoid areas (asterisks) of alveolar bone that remained unchanged in Hyp groups despite Spp1 −/− deletion. (E) Acellular cementum thickness remains diminished in all Hyp mice compared to controls. (F) Dynamic mechanical analysis testing reveals compromised dynamic stiffness (K * ) and energy dissipation (tan δ) in Hyp mice remain despite Spp1 ablation. AB: alveolar bone; CC: cellular cementum; DE: dentin; PDL: periodontal ligament; AC: acellular cementum. * p < .05; ** p < .01; *** p < .001; **** p < .0001.

    Journal: JBMR Plus

    Article Title: Genetic reduction or deletion of osteopontin in osteomalacic Hyp mice fails to ameliorate dentoalveolar defects

    doi: 10.1093/jbmrpl/ziaf170

    Figure Lengend Snippet: Persistent periodontal defects in Hyp groups despite Spp1 ablation. (A) H&E staining shows thin dentin (arrows) and wide pulp chamber compared to WT and Spp1 −/− controls. (B) Picrosirius red (PR) staining demonstrates that all Hyp groups have disorganized and detached PDL fibers at root surfaces (asterisks), compared to controls. (C) Immunohistochemistry (IHC) for osteopontin (OPN) shows accumulation of the protein around the osteocytes in alveolar bone (triangles) of Hyp and Hyp;Spp1 −/+ . Absence of OPN is apparent in Spp1 −/− and Hyp;Spp1 −/− mice. The graph in (D) IHC specific to bone sialoprotein (BSP) reveals unmineralized osteoid areas (asterisks) of alveolar bone that remained unchanged in Hyp groups despite Spp1 −/− deletion. (E) Acellular cementum thickness remains diminished in all Hyp mice compared to controls. (F) Dynamic mechanical analysis testing reveals compromised dynamic stiffness (K * ) and energy dissipation (tan δ) in Hyp mice remain despite Spp1 ablation. AB: alveolar bone; CC: cellular cementum; DE: dentin; PDL: periodontal ligament; AC: acellular cementum. * p < .05; ** p < .01; *** p < .001; **** p < .0001.

    Article Snippet: Immunohistochemistry (IHC) was performed using an avidin/biotin-based peroxidase immunostaining kit (Vectastain Elite ABC-HRP kit, Vector Laboratories) to produce a red-brown product ( n = 5/group for each target).

    Techniques: Staining, Immunohistochemistry